This study investigates the explicit and implicit biases favoring men as leaders, among both men and women faculty, and assesses whether these attitudes change following an educational intervention. 281 faculty members participated in a standardized, 20-minute educational course on implicit biases and strategies for overcoming them. Results indicated that the intervention significantly changed all faculty members’ perceptions of bias. These results suggest that providing education can serve as an important step toward reducing gender bias in academic medicine and, ultimately, promote institutional change, specifically the promotion of women to higher ranks.