This longitudinal cohort study, published in Education for Primary Care, seeks to identify which specific general practice trainee groups are particularly at risk of burnout and the aspects of training they find stressful. The results indicate higher than anticipated levels of burnout in all trainees. A sub-group self reporting higher levels of burnout comprised all-female, UK-trained-at-undergraduate general practice trainees with a partner but no children. Top reported stressors are uncertainty, workload, time pressures and ePortfolio. Less than 50% of trainees perceived their burnout levels to be as high as measured, revealing a potential lack of insight. Due to high levels of burnout as early as the first year of training, the authors advise early identification of burnout to help protect the future GP workforce.